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Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping.

Jiazhang Lian1,2, Carl Schultz1, Mingfeng Cao1

  • 1Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

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|December 21, 2019
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Summary
This summary is machine-generated.

Researchers developed a multi-functional genome-wide CRISPR (MAGIC) system for precise gene expression control. This powerful tool enables the creation of diverse genomic libraries to uncover complex genetic interactions and engineer cellular functions.

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Area of Science:

  • Synthetic biology
  • Genomics
  • Molecular biology

Background:

  • Understanding cellular networks requires genome-scale engineering.
  • Current methods for genome-wide genotype-phenotype mapping are limited to single genomic alteration modes (e.g., overexpression, repression, deletion).

Purpose of the Study:

  • To develop a versatile system for precise, genome-wide control of gene expression levels.
  • To create a comprehensive and diversified genomic library for functional genomics studies.

Main Methods:

  • Development of the multi-functional genome-wide CRISPR (MAGIC) system.
  • Integration of a tri-functional CRISPR system with array-synthesized oligonucleotide pools.
  • Application in yeast for creating a large-scale genomic library.

Main Results:

  • Demonstrated precise control of gene expression levels across the entire genome.
  • Generated one of the most comprehensive and diversified genomic libraries in yeast.
  • Identified novel genetic determinants of complex phenotypes, including synergistic interactions.

Conclusions:

  • The MAGIC system is a powerful synthetic biology tool for investigating fundamental biological questions.
  • MAGIC facilitates the engineering of complex phenotypes for biotechnological applications.
  • This system overcomes limitations of existing methods for genotype-phenotype mapping.