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Updated: Jan 1, 2026

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
Published on: December 20, 2021
Raman Trapping Microscopy for Non-invasive Analysis of Biological Samples
Hesham K Yosef1, Karin Schütze2
1CellTool GmbH, Tutzing, Germany. h.yosef@celltool.de.
Abstract:
Raman microscopy is an emerging tool in biomedicine. It provides label-free and non-invasive analysis of biological cells. Due to its high biochemical specificity, Raman spectroscopy can be used to acquire spectral fingerprints that allow characterizing cells types and states. Here, we present a methodological approach for implementing Raman microscopy in skin cell measurements. Raman spectra can clearly identify keratinocytes, fibroblasts, and melanocytes cells that are involved in the production of autologous skin grafts. Consequently, Raman microscopy is a promising tool that can be used to analyze single cells and to test the quality of therapeutic cell products.
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