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Long noncoding RNA SNHG14 exerts oncogenic functions in lung adenocarcinoma through acting as a sponge to miR-613
1Department of Thoracic Surgery, China-Japan Union Hospital of Jilin University, Changchun, China. sunhb2003@163.com.
Objective:
Lung adenocarcinoma is one of the most ordinary malignant tumors. Recent researches have proved that long noncoding RNAs (lncRNAs) are vital factors in many diseases. In this work, lncRNA SNHG14 was studied to identify its function in the development of lung adenocarcinoma.
Patients And Methods:
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect SNHG14 expression in paired lung adenocarcinoma patients' tissue samples and cells. Then, the function of SNHG14 was detected through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, colony formation assay, and transwell assay in vitro. Besides, mechanism assays and the interaction between SNHG14 and miR-613 were conducted.
Results:
SNHG14 was remarkably higher-expressed in lung adenocarcinoma tissues than in adjacent samples. Moreover, cell proliferation and invasion of lung adenocarcinoma were promoted via overexpression of SNHG14, while cell proliferation and invasion of lung adenocarcinoma were inhibited via silence of SNHG14. Moreover, RT-qPCR results revealed that miR-613 was downregulated via overexpression of SNHG14, while miR-613 was upregulated via knockdown of SNHG14. Further experiments showed that miR-613 was also a direct target of SNHG14 in lung adenocarcinoma.
Conclusions:
Our study suggests that SNHG14 enhances lung adenocarcinoma cell proliferation and invasion via targeting miR-613, which indicates that SNHG14 may be a potential therapeutic target in lung adenocarcinoma.
Insights
Long noncoding RNA SNHG14 promotes lung adenocarcinoma cell growth and spread by targeting miR-613. This suggests SNHG14 is a potential therapeutic target for lung adenocarcinoma treatment.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) play crucial roles in various diseases.
- Lung adenocarcinoma is a prevalent malignant tumor with complex molecular underpinnings.
Purpose of the Study:
- To investigate the function of lncRNA SNHG14 in the development of lung adenocarcinoma.
- To elucidate the molecular mechanism by which SNHG14 influences lung adenocarcinoma progression.
Main Methods:
- Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) to assess SNHG14 expression.
- In vitro assays including MTT, colony formation, and transwell assays to evaluate cell proliferation and invasion.
- Mechanism assays to determine the interaction between SNHG14 and miR-613.
Main Results:
- SNHG14 expression was significantly elevated in lung adenocarcinoma tissues compared to adjacent samples.
- Overexpression of SNHG14 promoted, while silencing inhibited, lung adenocarcinoma cell proliferation and invasion.
- SNHG14 regulated miR-613 expression, with SNHG14 directly targeting miR-613 in lung adenocarcinoma.
Conclusions:
- SNHG14 enhances lung adenocarcinoma cell proliferation and invasion by targeting miR-613.
- SNHG14 represents a potential therapeutic target for lung adenocarcinoma.
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