Related Experiment Videos
GABA-mediated inhibition in nucleus paragigantocellularis lateralis in the cat
1Department of Physiology, Medical School, Birmingham, U.K.
Abstract:
In decerebrate cats electrical stimulation (10-20 ms trains, 35-450 microA, 0.1 ms pulses, 450 Hz) in nucleus tractus solitarius (NTS, medial or lateral divisions) or nucleus raphe magnus (NRM) inhibited spontaneous or amino acid-induced neuronal activity in nucleus paragigantocellularis lateralis (PGL) for 8-140 (mean 51) ms. Iontophoretically applied GABA (2-50 nA) also inhibited these cells. Iontophoretically applied bicuculline methiodide (10-80 nA) blocked the effects of GABA and reduced the duration of the inhibition evoked from NRM by greater than 50% (5/6 cells) but had no effect on the inhibition evoked from NTS (6/7 cells). The results are discussed in relation to the role of GABA in mediating inhibitory afferent input to PGL.