Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid and the eye lens.

S D Varma1, R D Richards

  • 1Department of Ophthalmology, School of Medicine, University of Maryland, Baltimore.

Ophthalmic Research
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of MBE growth conditions on GaAsBi photoluminescence lineshape and localised state filling.

Scientific reports·2022
Same author

Analysis of Bi Distribution in Epitaxial GaAsBi by Aberration-Corrected HAADF-STEM.

Nanoscale research letters·2018
Same author

Oxidative stress in lens in vivo: inhibitory effect of caffeine. A preliminary report.

Molecular vision·2010
Same author

Intraocular penetration of pyruvate following its topical administration in mice.

Molecular and cellular biochemistry·2009
Same author

Electron impact mass spectroscopic studies on mouse retinal fatty acids: effect of diabetes.

Ophthalmic research·2009
Same author

Possibility of topical antioxidant treatment of cataracts: corneal penetration of pyruvate in humans.

Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde·2008
Same journal

Retraction Statement.

Ophthalmic research·2026
Same journal

Correlations between Clinical Activity Score and Serological and Radiological Data in Treatment-naïve Ethnic Han Chinese Thyroid Eye Disease.

Ophthalmic research·2026
Same journal

Stem Cell Based Interventions for Retinal Ganglion Cell Protection and Regeneration in Glaucoma: A Review of Current Evidence and Future Directions.

Ophthalmic research·2026
Same journal

Eye-Tracking Reading Patterns as Correlates of Clinical Measures in Glaucoma.

Ophthalmic research·2026
Same journal

A Matching-Adjusted Comparison of Faricimab and Aflibercept 8 mg in Neovascular Age-Related Macular Degeneration and Diabetic Macular Edema.

Ophthalmic research·2026
Same journal

Ab Externo Continuous Pressure Technique for Descemet Membrane Endothelial Keratoplasty in Vitrectomized Eyes.

Ophthalmic research·2026
See all related articles

Hyperbaric oxygen exposure causes lens cataracts in mice by generating damaging oxygen radicals. Antioxidants like ascorbate may prevent this, suggesting a role in combating cataracts.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Hyperbaric oxygen (HBO) exposure induces lens mitotic inhibition and cataracts in mice.
  • Similar cataracts are observed in humans undergoing HBO therapy.
  • Lens damage is hypothesized to result from in situ reactive oxygen species (ROS) generation.

Purpose of the Study:

  • To investigate the role of ROS in HBO-induced lens damage and cataract formation.
  • To evaluate the protective effects of antioxidants against ROS-induced lens injury.
  • To explore the potential anti-cataract effects of ascorbate and pyruvate.

Main Methods:

  • In vitro experiments using rat lenses cultured in a medium generating oxygen radicals (photochemically or enzymatically).
  • Assessment of lens tissue physiology by measuring rubidium and amino acid transport.

Related Experiment Videos

  • Evaluation of protective effects of ROS scavengers, ascorbate, and pyruvate.

    • Main Results:

    • HBO exposure leads to inhibition of mitotic activity and cataract development in the mouse lens.
    • In vitro experiments demonstrated adverse effects on lens transport functions due to ROS.
    • ROS scavengers, ascorbate (at physiological concentrations), and pyruvate showed protective effects against lens damage.

    Conclusions:

    • In situ generation of ROS is a key factor in HBO-induced lens damage and cataract formation.
    • Ascorbate and pyruvate exhibit antioxidant and potential anti-cataract properties.
    • These findings suggest a link between oxidative stress and senile cataractogenesis, with potential therapeutic implications for antioxidants.