Transcriptome analysis-identified long noncoding RNA CRNDE in maintaining endothelial cell proliferation, migration,...

Area of Science:

• Molecular Biology
• Endocrinology
• Cardiovascular Research

Background:

• Obesity is a major risk factor for type-2 diabetes, which can disrupt angiogenesis.
• Long noncoding RNAs (lncRNAs) are known to influence angiogenesis, but their role in obesity-related diabetes complications is unclear.

Purpose of the Study:

• To investigate how lncRNA expression changes in endothelial cells under obesity-like conditions induced by palmitic acid.
• To identify specific lncRNAs involved in regulating endothelial cell function in response to palmitic acid.

Main Methods:

• Endothelial cells were treated with excessive palmitic acid to mimic obesity.
• Bioinformatics analysis was used to identify differentially expressed protein-coding transcripts and lncRNAs.
• Gene ontology and pathway analysis were performed.
• The lncRNA CRNDE was selected for functional investigation, including knockdown experiments.

Main Results:

• Palmitic acid treatment altered the expression of numerous protein-coding transcripts and lncRNAs in endothelial cells.
• Enriched pathways included endoplasmic reticulum stress, HIF-1 signaling, AGE-RAGE signaling, and IL-17 signaling.
• The lncRNA CRNDE was upregulated by palmitic acid and its knockdown impaired endothelial cell proliferation, migration, and capillary tube formation.

Conclusions:

• lncRNA CRNDE plays a significant role in regulating endothelial cell behavior under palmitic acid stress.
• CRNDE is a potential therapeutic target for managing angiogenesis dysregulation in obesity-induced diabetes complications.