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Related Experiment Video

Updated: Jan 1, 2026

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
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Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip.

Hiroaki Takehara1, Katsuhisa Sakaguchi2, Hidekazu Sekine3

  • 1Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. takehara@bionano.t.u-tokyo.ac.jp.

Biomedical Microdevices
|December 22, 2019
PubMed
Summary

A new microfluidic vascular-bed (micro-VB) device enables 3D tissue engineering with functional vascular networks. This platform facilitates seamless integration of engineered tissues with fluidic systems for regenerative medicine applications.

Keywords:
Artificial vesselsDrug screeningEndothelial cellsPetri dishRegenerative medicineVascularization

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • 3D tissue engineering aims to create functional tissues for regenerative medicine.
  • Developing perfusable vascular networks within engineered tissues remains a significant challenge.
  • Existing methods often lack seamless integration with external fluidic systems.

Purpose of the Study:

  • To introduce a novel microfluidic vascular-bed (micro-VB) device.
  • To demonstrate its capability for creating vascularized 3D engineered tissues.
  • To facilitate functional connections between engineered capillaries and macroscopic fluidic channels.

Main Methods:

  • Co-culture of human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) in fibrin gel.
  • Cellular self-assembly on the micro-VB device to form endothelial networks.
  • Perfusion of fluorescent microspheres to confirm vascular connection and functionality.
  • Integration of the micro-VB device within standard Petri dishes.

Main Results:

  • Successful formation of endothelial networks within 3D engineered tissues.
  • Functional connections established between endothelial capillaries (5-100 μm) and microfluidic channels (1-10 mm).
  • Demonstrated compatibility with standard cell culture equipment and procedures.
  • Confirmation of fluid flow through the engineered vascular system.

Conclusions:

  • The micro-VB device provides a versatile platform for vascularized 3D tissue engineering.
  • It enables the creation of functional vascular networks mimicking physiological conditions.
  • This technology is expected to enhance the routine application of 3D tissue engineering in regenerative medicine.