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Updated: Jan 1, 2026
Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples
Published on: March 3, 2017
Label-free serum detection of Trichinella spiralis using surface-enhanced Raman spectroscopy combined with
Jian Li1, Jing Ding1, Xiaolei Liu1
1Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Abstract:
Based on blood serum surface-enhanced Raman spectroscopy (SERS) analysis, this paper proposed a simple and unlabeled non-invasive serum detection for T. spiralis infection. Serum samples were collected and analyzed from 40 rats at 0 days post infection (dpi) (normal rats), 19 uninfected rats, and 16 rats infected with T. spiralis at 28 dpi, using SERS measurements. Multivariate statistical techniques, such as linear discriminant analysis (LDA) and principal components analysis (PCA), were used to analyze and identify the obtained blood serum SERS spectra. The diagnosis algorithms, based on PCA-LDA, achieved a diagnostic sensitivity of 87.5%, a specificity of 94.7%, and an accuracy of 91.4% for separating the samples infected with T. spiralis from the control samples. This exploratory study demonstrated that colloidal Ag NPs-based SERS serum analysis technique combined with PCA-LDA has a great potential in improving the detection of T. spiralis infection and onsite screening.
