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Updated: Jan 1, 2026

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
Published on: July 12, 2022
Data on heteroplasmic mutations in mitochondrial genomes of loggerhead and hawksbill sea turtles: First approach
David Delgado-Cano1, Leonardo Mariño-Ramirez2, Javier Hernández-Fernández1
1Department of Natural and Environmental Science, Marine Biology Program, Faculty of Science and Engineering, Genetics, Molecular Biology and Bioinformatics Research Group -GENBIMOL, Jorge Tadeo Lozano University, Cra. 4 No 22-61, Bogotá, Colombia.
Abstract:
The populations of loggerhead (Caretta caretta) and hawksbill (Eretmochelys imbricata) sea turtles are suffering an exponential decline due to anthropic and environmental actions that threaten their survival. In these turtle populations, the degree of heteroplasmic mutations commonly related with pathologies, has not been studied. In this data report, the specifications of each heteroplasmic site (region, mutation, length) and the percentage of heteroplasmy of each gene for four mitochondrial genomes of turtles (loggerhead: Cc1, Cc2, Cc3 and hawksbill: Ei1) are presented. The highest value of heteroplasmy in tRNA was of 83.33% for the Cc2 turtle (tRNASer gene), in protein coding genes was 38.62% for Cc2 (ND5), and in rRNA genes of 0.74% for Ei1 turtle (rRNA-16S). The variability data obtained will be useful for further conservation projects, evolution studies and population health of these species. This is the first study of heteroplasmy in complete mitogenomes of loggerhead and hawksbill turtles.
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