Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational design and synthesis of TBC1D2 inhibitors: Augmenting autophagy to improve sorafenib sensitivity in hepatocellular carcinoma.

European journal of medicinal chemistry·2026
Same author

Chronic intermittent hypoxia sustains the low-grade inflammatory memory of monocytes via activation of myelopoiesis.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026
Same author

Effects of perinatal depression on early breastfeeding: the role of childbirth experience.

BMC pregnancy and childbirth·2026
Same author

Metal-regulated MXene nanozyme with second near-infrared-triggered photothermal and chemodynamic synergy for anti-infection therapy and transcriptomic deciphering of antibacterial mechanisms.

Journal of colloid and interface science·2026
Same author

PFKFB3 Mediated Glycolytic Reprogramming Drives Vascular Endothelial Injury Under Chronic Intermittent Hypoxia.

International journal of biological sciences·2026
Same author

Automatic pain assessment from facial action units in ICU patients via various machine learning models.

Scientific reports·2026

Related Experiment Video

Updated: Jan 1, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

16.2K

Three-dimensional ring-oven washing technique for a paper-based immunodevice.

Weiru Chu1, Ying Chen1, Wei Liu1

  • 1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, China.

Luminescence : the Journal of Biological and Chemical Luminescence
|December 24, 2019
PubMed
Summary

A novel 3D washing strategy for paper-based immudevices significantly reduces background noise by utilizing gravity and capillary action for efficient removal of nonspecific binding. This method enhances assay sensitivity, achieving a low detection limit for carcinoembryonic antigen.

Keywords:
ELISAchemiluminescencenonspecific-bindingpaper-based chipthree-dimensional washing

More Related Videos

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
07:53

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

Published on: April 1, 2016

7.9K
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds
08:16

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Published on: April 2, 2018

12.2K

Related Experiment Videos

Last Updated: Jan 1, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

16.2K
Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
07:53

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

Published on: April 1, 2016

7.9K
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds
08:16

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Published on: April 2, 2018

12.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Washing is crucial in paper-based enzyme-linked immunosorbent assays (ELISA) to eliminate non-specific binding.
  • Traditional washing methods on paper chips can be inefficient, leading to higher background signals.

Purpose of the Study:

  • To develop and evaluate a novel three-dimensional (3D) washing strategy for paper-based immudevices.
  • To improve the efficiency of removing non-specific binding antibodies and antigens from paper surfaces.

Main Methods:

  • A 3D washing strategy was implemented on a paper-based chip using a heating ring-oven and a 3D plastic holder.
  • Gravity and capillary action were leveraged to transport unbound substances to a heated waste area.
  • Upconversion fluorescence and chemiluminescence (CL) were used to assess washing effectiveness.

Main Results:

  • The 3D washing strategy demonstrated a lower background signal compared to conventional planar washing.
  • The method effectively removed non-specific binding proteins, as confirmed by fluorescence and CL imaging.
  • A lower detection limit of 2 pg/mL was achieved for carcinoembryonic antigen detection using CL.

Conclusions:

  • The novel 3D washing strategy offers an effective approach for enhancing the performance of paper-based immunodevices.
  • This method improves the removal of non-specific binding, leading to more sensitive and reliable diagnostic assays.