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Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles.
M P Prylutskyi1, M F Starodub2
1National University of Kyiv-Mohyla Academy, Kyiv 04070, Ukraine.
Experimental Oncology
|December 24, 2019
Summary
A novel zinc oxide nanoparticle biosensor effectively detects spermine and spermidine, crucial biogenic polyamines, in solutions and cell cultures. This advancement offers a promising tool for quantifying polyamines in biological samples.
Area of Science:
- Nanotechnology
- Biochemistry
- Biosensing
Background:
- Biogenic polyamines like spermine and spermidine play critical roles in cellular processes.
- Accurate detection of polyamines is essential for understanding various physiological and pathological conditions.
Purpose of the Study:
- To evaluate a biosensor utilizing zinc oxide nanoparticles for detecting spermine and spermidine.
- To assess the biosensor's performance in both model solutions and biological samples (MCF-7 cell lyophilisate).
Main Methods:
- Development of a biosensor incorporating zinc oxide nanoparticles and antibodies specific to spermine and spermidine.
- Measurement of polyamine concentration via laser-induced luminescence (380 nm) of the nanoparticles.
- Analysis of polyamine levels in both standard solutions and MCF-7 cell lyophilisate.
Main Results:
- The biosensor demonstrated detection limits for polyamines in model solutions ranging from 10 ng/ml to 100 ng/ml.
- In MCF-7 cell lyophilisate, the biosensor enabled detection of polyamines across a concentration range of 100 to 100,000 cells/ml.
- Enhanced luminescence intensity was observed when analyzing polyamines in cell lyophilisate.
Conclusions:
- The zinc oxide nanoparticle-based biosensor shows significant promise for the detection and measurement of biogenic polyamines.
- The developed system offers a viable approach for quantifying polyamines in biological systems, particularly in cell lyophilisate.
- Further refinement of this methodology could lead to improved diagnostic and research tools.
