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Electrorheological Sensor Encapsulating Microsphere Media for Plague Diagnosis with Rapid Visualization.

Pai-Chien Chou1,2, Feng-Ping Lin2,3, Hui-Ling Hsu3

  • 1Department of Thoracic Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan, Republic of China.

ACS Sensors
|December 24, 2019
PubMed
Summary

This study developed a novel electrorheological sensor for rapid, on-site plague diagnosis. The sensor detects Yersinia pestis using antibody-modified microspheres, offering high sensitivity and selectivity without fluorescence labeling.

Keywords:
Fe3O4core/shellelectrorheologymicrosphereparticle chainingplague

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Area of Science:

  • Biomaterials Science
  • Biosensing Technology
  • Electrorheology

Background:

  • Plague, caused by Yersinia pestis, requires rapid field diagnosis due to its transmission via fleas to rodents.
  • Existing diagnostic methods can be time-consuming and complex, hindering immediate on-site detection.

Purpose of the Study:

  • To develop a sensitive and selective biosensor for rapid, on-site detection of Yersinia pestis.
  • To utilize electrorheological displays (EDs) for visualizing plague pathogen presence without fluorescence labeling.

Main Methods:

  • Synthesis of core-shell structured polystyrene microspheres (SMs) adsorbing magnetic nanoparticles (FNs).
  • Construction of electrorheological displays (EDs) containing SM@FN solutions.
  • Modification of SM@FN microspheres with antibodies against Yersinia pestis (ab-Yp) for antigen capture.
  • Monitoring changes in characteristic frequency (Fc) and transmittance in response to Y. pestis antigen (ag-Yp).

Main Results:

  • The electrorheological sensor demonstrated a significant shift in characteristic frequency (Fc) from 200 to 750 kHz upon Y. pestis antigen detection.
  • High sensitivity was achieved with a limit of detection around 30 ng/μL within 30 seconds.
  • The sensor showed high selectivity, unaffected by common bacteria like Escherichia coli and Staphylococcus aureus.

Conclusions:

  • The developed electrorheological sensor offers a simple, sensitive, and selective platform for on-site plague diagnosis.
  • This technology bypasses the need for fluorescence labeling, enabling direct visual detection.
  • The sensor presents a promising alternative to conventional immunoassays for rapid field identification of Yersinia pestis.