Cytotoxicity of vanadium oxide nanoparticles and titanium dioxide-coated vanadium oxide nanoparticles to human lung...

Due to excellent metal-insulator transition property, vanadium dioxide nanoparticles (VO₂ NPs)-based nanomaterials are extensively studied and applied in various fields, and thus draw safety concerns of VO₂ NPs exposure through various routes. Herein, the cytotoxicity of VO₂ NPs (N-VO₂ ) and titanium dioxide-coated VO₂ NPs (T-VO₂ ) to typical human lung cell lines (A549 and BEAS-2B) was studied by using a series of biological assays. It was found that both VO₂ NPs induced a dose-dependent cytotoxicity, and the two cell lines displayed similar sensitivity to VO₂ NPs. Under the same conditions, T-VO₂ NPs showed slightly lower cytotoxicity than N-VO₂ in both cells, indicating the surface coating of titanium dioxide mitigated the toxicity of VO₂ NPs. Titanium dioxide coating changed the surface property of VO₂ NPs and reduced the vanadium release of particles, and thus helped lowing the toxicity of VO₂ NPs. The induced cell viability loss was attributed to apoptosis and proliferation inhibition, which were supported by the assays of apoptosis, mitochondrial membrane damage, caspase-3 level, and cell cycle arrest. The oxidative stress, i.e., enhanced reactive oxygen species generation and suppressed reduced glutathione , in A549 and BEAS-2B cells was one of the major mechanisms of the cytotoxicity of VO₂ NPs. These findings provide safety guidance for the practical applications of vanadium dioxide-based materials.