Related Experiment Video
Updated: Jan 1, 2026
Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies
Published on: February 17, 2023
Lipid and poly (ethylene glycol)-conjugated bi-functionalized chlorine e6 micelles for NIR-light induced photodynamic
Omkara Swami Muddineti1, Sri Vishnu Kiran Rompicharla1, Preeti Kumari1
1Department of Pharmacy, Birla Institute of Technology & Science Pilani, Hyderabad Campus, Jawahar Nagar, Medchal, Hyderabad, 500078, Telangana, India.
Background:
To develop a photosensitizer, chlorin e6 (Ce6)-based amphiphilic polymer, DP-Ce6, where DOPE and PEG are conjugated to Ce6, which would self-assemble to form polymeric micelles (DP-Ce6-M) in aqueous environment.
Methods:
DP-Ce6-M were characterized for particle size, zeta potential, and singlet oxygen (1O2) generation. Cellular internalization, phototoxicity were investigated against monolayer and 3D spheroids of human lung adenocarcinoma cells (A549).
Results And Conclusions:
DP-Ce6-M formed stable micelles with particles size of 58.2 ± 1.6 nm. Solubility of Ce6 was improved. Photoactivity of DP-Ce6-M was sustained in regard to 1O2 generation compared to free Ce6. The DP-Ce6-M showed enhanced internalization and growth inhibition in monolayer and spheroidal cells. Overall, DP-Ce6-M demonstrated the potential for further exploration as PDT agent for cancer treatment.
