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Published on: March 2, 2017
[Cloning,subcellular localization and expression pattern analysis of transcription factor SmGRAS3 from Salvia
Wen-Rui Li1, Zhen-Qing Bai2, Jing-Ling Liu2
1Institute of Soil and Water Conservation,the Ministry of Water Resources,Chinese Academy of Sciences Yangling 712100,China University of the Chinese Academy of Sciences Beijing 100049,China.
Abstract:
GRAS transcription factors play important roles in the regulation of plant root growth and GA signaling. In this study,SmGRAS3 gene was cloned,which open reading frame was 2 247 bp,and encoding 748 amino acids. The physicochemical properties and structure of SmGRAS3 and its encoded protein were analyzed by bioinformatics software. This gene belongs to the SCL9 subfamily of the GRAS family,and its promoter sequence mainly contains the light response,stress response,and hormone response elements. It may interact with the GA signal pathway and anti-stress related proteins. The subcellular localization showed that SmGRAS3 protein was mainly located in the nucleus. The expression pattern analysis showed that the expression of Sm GRAS3 was the highest in the root and the lowest in the stem,and both light and low temperature could induce the high expression level of SmGRAS3. This study provides a foundation for further study on the roles of SmGRAS3 gene in the root growth and stress tolerance of Salvia miltiorrhiza.
