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Updated: Jan 1, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
LncRNA miR143HG suppresses miR-21 through methylation to inhibit cell invasion and migration
1Department of Maxillofacial Surgery, Guangxi Medical University College of Stomatology, Nanning City, Guangxi Province, People's Republic of China.
Objective:
This study aimed to investigate the role of lncRNA miR143HG in laryngeal squamous cell carcinoma (LSCC).
Methods:
Quantitative polymerase chain reaction (PCR) and paired t test were used to measure and compare expression levels of miR143HG and miR-21 in LSCC and nontumor tissues. To analyze the interactions between miR143HG and miR-21, UM-SCC-17A cells were transfected miR143HG expression vector or miR-21 mimic. The effects of miR143HG and miR-21 overexpression on UM-SCC-17A cell invasion and migration were analyzed by transwell assays.
Results:
We found that miR143HG was downregulated in LSCC and inversely correlated with miR-21. In LSCC cells, miR143HG overexpression led to the downregulated expression of miR-21, whereas miR-21 overexpression failed to affect miR143HG. Methylation-specific PCR results showed that miR143HG overexpression led to increased methylation of miR-21. Low expression levels of miR143HG were correlated with poor survival. Overexpression of miR143HG led to decreased, whereas miR-21 overexpression resulted in increased rate of LSCC cell migration and invasion. In addition, miR-21 overexpression led to reduced effects of miR143HG on cell invasion and migration.
Conclusion:
Therefore, miR143HG suppresses miR-21 via methylation to regulate cell behaviors in LSCC.
Level Of Evidence:
NA Laryngoscope, 130:E640-E645, 2020.
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