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Related Experiment Video

Updated: Jan 1, 2026

Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model
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Generation of a hypoxia-sensing mouse model.

Qun Lin1, Yan Huang2, Frank J Giordano2

  • 1Department of Therapeutic Radiology, Yale School of Medicine, New Haven, Connecticut.

Genesis (New York, N.Y. : 2000)
|December 25, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel hypoxia sensor (O2 CreER) and mouse model (R26-O2 CreER) to track oxygen-sensing cells. This tool identifies hypoxic cells in adult mice and developing embryos, aiding hypoxia research.

Keywords:
Cre recombinaseROSA26hypoxiahypoxia-inducible factorischemiamTmGskinvibrissa

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Area of Science:

  • Physiology
  • Molecular Biology
  • Genetics

Background:

  • Oxygen (O2) homeostasis is critical for metazoan life.
  • Hypoxia-regulated molecular pathways are vital for cellular functions and survival.
  • Understanding O2 sensing is crucial for various biological processes.

Purpose of the Study:

  • To design an innovative hypoxia sensor (O2 CreER) for tracking oxygen-sensing cells.
  • To generate a novel hypoxia-sensing mouse model (R26-O2 CreER) for in vivo studies.
  • To provide a valuable genetic tool for studying hypoxia in mammals.

Main Methods:

  • Designed an O2 CreER hypoxia sensor using HIF-1α degradation domain and Cre recombinase.
  • Generated the R26-O2 CreER mouse model by inserting the O2 CreER cassette into the ROSA26 locus.
  • Utilized ROSA(mTmG) reporter mice to visualize hypoxic cell labeling.

Main Results:

  • The R26-O2 CreER mouse model specifically identified hypoxic cells in adult mice with hind-limb ischemia.
  • This model successfully labeled embryonic cells, including vibrissal follicle cells, during development (E13.5-E15.5).
  • Demonstrated the model's efficacy in both physiological and pathological conditions.

Conclusions:

  • The novel R26-O2 CreER mouse model is an effective tool for studying hypoxia.
  • This genetic tool enables precise identification of O2-sensing cells in various contexts.
  • Facilitates research into hypoxia and O2 sensing in mammalian systems.