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Updated: Jan 1, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
[Methods of Evaluating the Efficiency of CRISPR/Cas Genome Editing]
N A Lomov1,2,3, V S Viushkov1,2, A P Petrenko1
1Moscow State University, Moscow, 119991 Russia.
Abstract:
The CRISPR/Cas system is currently widely used for genome editing. The procedure of genome editing includes two necessary steps: (i) searching for the most effective guide RNA, and (ii) analyzing clones for presence of the desired mutation. This review presents the methods used to assess the efficiency of the CRISPR/Cas system and to confirm mutation in the target locus and discusses their advantages and disadvantages. It aims to provide information that could help researchers to choose a technique most appropriate for their specific tasks and available resources.
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