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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

3.2K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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Chemotaxonomic Profiling Through NMR1.

María José Iglesias1, Raquel Soengas1, Clara B Martins2

  • 1Área de Química Orgánica, Research Centre CIAIMBITAL, Universidad de Almería, Ctra. Sacramento s/n, 04120, Almería, Spain.

Journal of Phycology
|December 27, 2019
PubMed
Summary

Nuclear magnetic resonance (NMR) analysis revealed diverse metabolites in cyanobacteria. This screening identified specific lipids and compounds, aiding in chemotaxonomic classification of various strains.

Keywords:
ACOINMRchemotaxonomycyanobacteriametabolite identificationmorphology

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Area of Science:

  • Biochemistry
  • Phycology
  • Analytical Chemistry

Background:

  • Cyanobacteria are diverse microorganisms with potential for producing valuable compounds.
  • Metabolite profiling is crucial for understanding cyanobacterial biochemistry and chemotaxonomy.
  • Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for metabolite identification.

Purpose of the Study:

  • To screen and identify metabolites from 25 cyanobacterial strains using NMR.
  • To investigate the lipid profiles of different cyanobacterial orders and subsections.
  • To explore the potential of NMR for chemotaxonomic classification of cyanobacteria.

Main Methods:

  • Sequential extraction of cyanobacterial biomass with hexane, ethyl acetate, and methanol.
  • Analysis of extracts using proton (1H) and phosphorus-31 (31P) NMR spectroscopy.
  • Quantification of specific lipids like galactosyldiacylglycerols and identification of other metabolites.

Main Results:

  • Triacylglycerols were found in two Nostoc strains.
  • Monogalactosyldiacylglycerols and digalactosyldiacylglycerols were major lipids in ethyl acetate extracts.
  • Polar metabolites, including sucrose oligosaccharides and mycosporine-like amino acids, were identified in methanol extracts.
  • Heterocyst glycolipids were present in Nostocales, while sulphoquinovosyldiacylglycerols were absent in Microchaete tenera.
  • Phosphatidylglycerol was the main phospholipid, with phosphatidylcholine as a minor component.

Conclusions:

  • NMR analysis effectively differentiates cyanobacterial strains based on their metabolite profiles.
  • The identified metabolites provide valuable chemotaxonomic markers for cyanobacteria.
  • This study highlights the metabolic diversity within the studied cyanobacterial collection.