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Updated: Jan 1, 2026

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Published on: June 29, 2021
Functional changes of biomolecules and organocatalysts as a probe for pressure effects in solution
Thomas Weinbender1, Martin Hofmann1, Oliver Reiser1
1Institute of Organic Chemistry, University Regensburg Universitätsstr, 31, 93053 Regensburg, Germany.
Abstract:
High pressure acts as a mild and non-destructive activation mode for chemical reactions. However, in the context of organo-/biocatalysis, high pressure activation, has not been investigated systematically, although there are significant benefits such as rate acceleration, increased selectivity and the possibility of suppressing side product formation. The influence of hydrostatic pressure in solution on the catalytic performance of enzymes and small molecule organocatalysts such as amino acids, peptides, amines, cinchona alkaloids and thioureas is evaluated in this review, taking reactivity and selectivity as a probe to identify pressure effects on biomolecules.
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