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Correction: Sutthanont et al. Effectiveness of Herbal Essential Oils as Single and Combined Repellents Against <i>Aedes aegypti</i>, <i>Anopheles dirus</i> and <i>Culex quinquefasciatus</i> (Diptera: Culicidae). <i>Insects</i> 2022, <i>13</i>, 658.

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DNA Barcoding Subtropical Aphids and Implications for Population Differentiation.

Qiang Li1, Jun Deng1, Cui Chen1

  • 1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

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Summary
This summary is machine-generated.

This study builds the first DNA barcode library for subtropical aphids, aiding species identification and revealing potential new species. The library supports biodiversity research and pest management in agriculture and forestry.

Keywords:
DNA barcodingaphidsphylogenypopulation divergencespecies identification

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Area of Science:

  • Entomology
  • Molecular Biology
  • Bioinformatics

Background:

  • DNA barcoding is crucial for species identification but lacks comprehensive reference libraries for many groups, including understudied subtropical aphids.
  • Aphids are significant agricultural and forestry pests, necessitating better tools for their identification and study.

Purpose of the Study:

  • To construct the first extensive DNA barcode reference library for subtropical aphids.
  • To assess the efficacy of DNA barcoding for aphid species identification and population differentiation.
  • To evaluate various species delimitation methods for aphid barcoding.

Main Methods:

  • Sequencing 1581 aphid specimens across 143 morphospecies from subtropical regions.
  • Utilizing Automatic Barcode Gap Discovery (ABGD), Generalized Mixed Yule-Coalescent (GMYC), and Bayesian Poisson Tree Processes (bPTP) for species delimitation.
  • Analyzing genetic divergence with a 2% threshold and examining host plant sampling effects.

Main Results:

  • Established a comprehensive DNA barcode library for 143 subtropical aphid morphospecies.
  • Confirmed DNA barcoding's utility, with most species showing clear barcode gaps and a 2% genetic distance threshold being effective.
  • Identified ten morphospecies potentially representing distinct lineages due to host plant or geographic factors.

Conclusions:

  • The new DNA barcode library is a valuable resource for aphid research and pest management.
  • DNA barcoding effectively distinguishes aphid species and aids in understanding population divergence.
  • Host plant sampling scale influences the reliability of DNA barcoding for phytophagous insects.