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Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016
Published on: June 23, 2023
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Microbial Transformation of Licochalcones
Yina Xiao1, Fubo Han1, Ik-Soo Lee1
1College of Pharmacy, Chonnam National University, Gwangju 61186, Korea.
Molecules (Basel, Switzerland)
|December 28, 2019
Summary
Microbial transformation of licochalcones yielded ten new metabolites. This study expands the structural diversity of retrochalcones, aiding further biological investigations and understanding isomerization mechanisms.
Area of Science:
- Natural Product Chemistry
- Microbiology
- Synthetic Chemistry
Background:
- Licochalcones are natural compounds with potential biological activities.
- Microbial transformations offer a sustainable route to novel compound derivatives.
- Understanding retrochalcone isomerization is crucial for drug development.
Purpose of the Study:
- Investigate microbial transformation of licochalcones B, C, D, and H.
- Isolate and characterize novel metabolites produced by fungal strains.
- Determine the structures and absolute configurations of new compounds.
Main Methods:
- Utilized filamentous fungi Aspergillus niger and Mucor hiemalis for biotransformation.
- Employed chromatographic techniques for metabolite isolation.
- Characterized compounds using UV, IR, MS, NMR, CD, and ECD spectroscopy.
Main Results:
- Isolated ten new metabolites: one hydrogenated, three dihydroxylated, three epoxidized, and three glucosylated.
- Determined absolute configurations of dihydroxylated metabolites using ECD.
- Observed trans-cis isomerization in four metabolites, providing insights into the mechanism.
Conclusions:
- Fungal transformation effectively expands the structural diversity of retrochalcones.
- The study provides new insights into the trans-cis isomerization of retrochalcones.
- Generated novel compounds for future biological activity screening and drug discovery.
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