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Updated: Jan 1, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

CXCL5 Modified Nanoparticle Surface Improves CXCR2⁺ Cell Selective Internalization.

Roberta Cagliani^1,2, Francesca Gatto¹, Giulia Cibecchini^1,2

¹Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.

|December 28, 2019

Summary

This summary is machine-generated.

Chemokine-decorated silica nanoparticles precisely target immune cells. This novel approach enhances nanoparticle uptake and localization for improved biomedical applications.

Keywords:

chemokine receptors chemokines immune cells nanoparticles surface chemistry

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A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

Area of Science:

Nanotechnology
Immunology
Biomedical Engineering

Background:

Targeting specific cell populations with nanomaterials remains a significant challenge in biomedical applications.
Current strategies involve material modification or surface decoration with active molecules.

Purpose of the Study:

To develop and validate chemokine-decorated silica nanoparticles for precise targeting of CXCR2+ immune cells.
To investigate the cell binding and internalization efficiency of these modified nanoparticles.

Main Methods:

Covalent binding of the chemokine CXCL5 onto fluorescently labeled amino-functionalized SiO2 nanoparticles.
Characterization of physicochemical properties, including z-potential and immunogold labeling.
Analysis of cell binding and internalization in CXCR2+ THP-1 cells using flow cytometry and confocal microscopy.

Main Results:

CXCL5-modified nanoparticles demonstrated enhanced internalization in THP-1 cells, both with and without serum.
Internalization was reduced by pre-treatment with free CXCL5, confirming receptor-specific uptake.
Preferential targeting of CXCR2+ cells was shown by comparing uptake in THP-1 versus low-CXCR2 expressing HeLa cells.

Conclusions:

Chemokine-decorated nanomaterials offer a viable strategy for enhanced cellular uptake and precise localization.
This approach enables selective targeting of chemokine receptor-expressing cells, with potential benefits for immune-related pathological conditions.