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Updated: Jan 1, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Multi-lobe superoscillation and its application to structured illumination microscopy
Abstract:
Superoscillating function is a band-limited function that is locally oscillating faster than its highest Fourier component. In this work, we study and implement methods to generate multi-lobe optical superoscillating beams, with nearly constant intensity and constant local frequency. We generated superoscillating patterns having up to 12 sub-wavelength oscillations, with local frequency of 20% to 40% above the band-limit. We then test the potential application of these beams to super-resolution structured illumination microscopy. By utilizing the Moiré effect on a fluorescent grating, we have demonstrated experimentally resolution improvement over the conventional sinusoidal illumination. Our simulations show that structured illumination microscopy with super oscillating multi-lobe beams can provide more than twofold improvement in resolution, with respect to the classical diffraction limit and for coherent or incoherent modalities.
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