Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.6K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

969
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
969
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compact polarization-entangled source near 810 nm and its application to nonlocal retardance measurement.

Optics letters·2026
Same author

Single plane spatial mode sorter.

Optics express·2026
Same author

Structure and dynamics in the low-density phase of a two-dimensional cellular automaton model of traffic flow.

Physical review. E·2026
Same author

Effect of preferential node deletion on the structure of networks that evolve via preferential attachment.

Physical review. E·2025
Same author

Optically programable quasi phase matching in four-wave mixing.

Nature communications·2025
Same author

Pseudo-spin light circuits in nonlinear photonic crystals.

Nature communications·2025
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jan 1, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.0K

Multi-lobe superoscillation and its application to structured illumination microscopy.

Niv Shapira, Zhigui Deng, Roei Remez

    Optics Express
    |December 28, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Researchers generated optical superoscillating beams for enhanced microscopy. These beams enable faster-than-light oscillations, significantly improving resolution in super-resolution microscopy techniques.

    More Related Videos

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.9K

    Related Experiment Videos

    Last Updated: Jan 1, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.9K

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Wave Phenomena

    Background:

    • Superoscillating functions exhibit local oscillations exceeding their highest Fourier component.
    • Generating optical superoscillating beams with controlled properties is challenging.

    Purpose of the Study:

    • To develop and implement methods for generating multi-lobe optical superoscillating beams.
    • To investigate the application of these beams in super-resolution structured illumination microscopy.
    • To demonstrate experimental resolution enhancement using superoscillating beams.

    Main Methods:

    • Generation of multi-lobe optical superoscillating beams with nearly constant intensity and local frequency.
    • Implementation of superoscillating patterns with up to 12 sub-wavelength oscillations.
    • Experimental testing using the Moiré effect on a fluorescent grating for structured illumination microscopy.

    Main Results:

    • Successfully generated superoscillating beams with local frequencies 20%-40% above the band-limit.
    • Demonstrated experimental resolution improvement over conventional sinusoidal illumination microscopy.
    • Simulations indicate potential for more than twofold resolution improvement beyond the diffraction limit.

    Conclusions:

    • Optical superoscillating beams are promising tools for advancing super-resolution microscopy.
    • Superoscillating beams offer a pathway to overcome classical diffraction limits in imaging.
    • The developed methods pave the way for enhanced resolution in both coherent and incoherent imaging modalities.