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Anhydrous Hydrogen Fluoride Cleavage in Boc Solid Phase Peptide Synthesis.

Kirtikumar B Jadhav1, Katrina J Woolcock1, Markus Muttenthaler2

  • 1Faculty of Chemistry, Institute of Biological Chemistry, University of Vienna, Vienna, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|December 28, 2019
PubMed
Summary

This study details a protocol for anhydrous hydrogen fluoride (HF) cleavage, a critical step in tert-butyloxycarbonyl/benzyl chemistry solid-phase peptide synthesis (Boc-SPPS). The improved protocol enhances accessibility for producing peptides for research and clinical applications.

Keywords:
Boc chemistryHydrogen fluoride (HF) cleavageSolid phase peptide synthesis (SPPS)

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Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Chemical Synthesis

Background:

  • Solid-phase peptide synthesis (SPPS) is crucial for peptide production.
  • Tert-butyloxycarbonyl/benzyl chemistry (Boc-SPPS) is a widely used SPPS method.
  • Anhydrous hydrogen fluoride (HF) cleavage is a key step in Boc-SPPS for deprotection and resin cleavage.

Purpose of the Study:

  • To provide a detailed and accessible protocol for HF cleavage.
  • To improve the usability of this established technique.
  • To facilitate the production of peptides for research and clinical use.

Main Methods:

  • Detailed protocol for anhydrous hydrogen fluoride (HF) cleavage.
  • Focus on improving accessibility and user-friendliness.
  • Application within the context of tert-butyloxycarbonyl/benzyl chemistry solid-phase peptide synthesis (Boc-SPPS).

Main Results:

  • A refined protocol for HF cleavage is presented.
  • The protocol aims to enhance the accessibility of Boc-SPPS.
  • The technique remains valuable and well-validated for peptide synthesis.

Conclusions:

  • The provided HF cleavage protocol enhances the accessibility of a critical step in Boc-SPPS.
  • This detailed method supports the production of peptides for diverse applications.
  • The protocol reinforces the utility of HF cleavage in peptide synthesis.