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Updated: Jan 1, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Profiling Cell-Matrix Adhesion Using Digitalized Acoustic Streaming.

Hongwei Cai¹, Zheng Ao¹, Zhuhao Wu¹

¹Department of Intelligent Systems Engineering , Indiana University , Bloomington , Indiana 47405 , United States.

Analytical Chemistry

|December 28, 2019

Summary

This summary is machine-generated.

Researchers developed a novel acoustofluidic device to measure single-cell adhesion forces. This technology profiles cell-matrix interactions, aiding cancer metastasis research and diagnostics.

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Area of Science:

Biophysics
Cell Biology
Biomaterials

Background:

Cell-matrix adhesion is crucial for physiological and pathological processes, including development, healing, and cancer metastasis.
Accurate profiling of cell adhesion kinetics at the single-cell level is essential for understanding these processes.

Purpose of the Study:

To develop a novel digital acoustofluidic device for parallel, single-cell level profiling of cell-matrix adhesion kinetics.
To demonstrate the device's utility in studying cancer cell metastasis by measuring adhesion forces to fibronectin.

Main Methods:

A digital acoustofluidic device was engineered using localized acoustic streaming in an open microfluidic chamber.
Acoustic streaming-induced Stokes drag force was employed to detach cells from fibronectin-coated substrates.
Pulsed acoustic power regulated the detachment of hundreds of cells, enabling measurement of adhesive forces (pN to nN) and kinetics.

Main Results:

The device successfully measured single-cell adhesion forces and kinetics with pN resolution.
Adhesion strength and kinetics of malignant (MCF-7, MDA-MB-231) and nonmalignant (MCF-10A) breast cancer cells were compared.
The technique differentiated metastatic potentials based on cell-substrate rupture forces.

Conclusions:

The developed acoustofluidic device offers a simple, operable method for parallel measurement of hundreds of single-cell adhesion forces.
This technology holds significant potential for fundamental cell biology research, cancer diagnostics, and tissue engineering.
The device enables precise quantification of cell-matrix interactions, advancing our understanding of cellular behavior in health and disease.