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Correlation between the surface hydrophobicities and elution orders of elapid neurotoxins and cardiotoxins on
G Osthoff1, A I Louw, C J Reinecke
1National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria, Republic of South Africa.
Abstract:
Hydrophilic and hydrophobic regions were predicted for Elapid neuro- and cardiotoxins. The contribution of these regions to the retention times of neuro- and cardiotoxins on hydrophobic-interaction HPLC was assessed from the known surface accessibilities of amino acid side-chains within these regions. Differences in retention times between neuro- and cardiotoxins on hydrophobic-interaction HPLC could be attributed to differences in hydrophobicity of regions 6-12 and 22-26 between these two types of toxins. Smaller differences in retention times between cardiotoxins were due to the variable hydrophobicities of regions 1-4 and 26-36.