Urinary atrazine metabolites as indicators for rat and human exposure to atrazine

Rats were given atrazine (2-chloro-4-ethylamino-6-(isopropylamino)-s-triazine) in drinking water for 1 or 3 weeks at 0.1 (0.45 mM), 0.2 (0.9 mM) or 0.5 g/l (2.3 mM) concentrations of the commercial agent. They excreted at both time points as the principal metabolite 2-chloro-4-ethylamino-6-amino-s-trazine in a dose-dependent fashion. The same urine test was applied on 6 railway men engaged in the weeding operation of railway lines with known atrazine exposure measured with hygienic techniques in their breathing zone. The spectrum of their atrazine metabolites was comprised of fully N-dealkylated atrazine and 2-chloro-4-ethylamino-6-amino-s-triazine. The sum of the two urinary metabolites reflected quantitatively the exposure.