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Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway

Xing Zhen1, Hu-Nan Sun2, Ren Liu1

  • 1Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju, Republic of Korea.

In Vivo (Athens, Greece)
|December 29, 2019
PubMed
Abstract

Insights

Plasma-activated medium (PAM) effectively inhibits pancreatic cancer cell growth and migration. PAM induces cancer cell death through reactive oxygen species (ROS)-dependent autophagy, showing potential as a novel pancreatic cancer therapy.

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Cell Biology

Background:

  • Non-thermal plasma treatment, utilizing plasma-activated medium (PAM), is an emerging technology for cancer therapy.
  • Previous studies demonstrated PAM's efficacy across various cancer cell lines.

Purpose of the Study:

  • To investigate the inhibitory effects of PAM on Aspc1 pancreatic cancer cells.
  • To elucidate the mechanisms underlying PAM-induced cell death in pancreatic cancer.

Main Methods:

  • Colony-formation, sphere-formation, wound-healing, and transwell assays were employed.
  • Immunocytochemistry and Western blot analyses were conducted to assess cellular changes.
  • Reactive oxygen species (ROS) levels and mitochondrial membrane potential were measured.

Main Results:

  • PAM exhibited a significant cytotoxic effect on Aspc1 cells compared to normal cells.
  • PAM inhibited colony and sphere formation, as well as cell migration in Aspc1 cells.
  • PAM induced ROS accumulation, reduced mitochondrial membrane potential, and triggered ROS-dependent autophagy, down-regulating the AKT/STAT3 pathway.

Conclusions:

  • PAM demonstrates potent anti-cancer effects on Aspc1 pancreatic cancer cells.
  • The mechanism involves ROS-dependent autophagy, leading to apoptosis.
  • PAM shows promise as a potential therapeutic agent for pancreatic cancer.
Keywords:
Non-thermal plasmaapoptosisautophagypancreatic cancerreactive oxygen species

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