[Isolation and properties of lysyl-tRNA-synthetase from rabbit skeletal muscles]

A method is developed to isolate lysyl-tRNA-synthetase from 93-95%-purity postribosomal supernatant fraction of skeletal muscle homogenate in rabbit. Novelty of the method is the ATP usage for muscle homogenization, which permits shortening the number of operations during the enzyme isolation. The molecular weight of protein is 68 +/- 10 kDa, the monomer unit consists of 540 amino acids and contains a carbohydrate component.