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Induced Osteogenesis in Plants Decellularized Scaffolds.

Jennifer Lee1, Hyerin Jung2,3, Narae Park2,3

  • 1Divison of Rheumatology, Department of Internal Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06281, Republic of Korea.

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|December 29, 2019
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Summary

Plant-derived cellulose scaffolds can generate bone-like tissue from human induced pluripotent stem cells (hiPSCs). Apple scaffolds with specific pore sizes showed promise for bone regeneration in animal models.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Developing in vitro bone-like tissue requires 3D culture systems mimicking the in vivo microenvironment.
  • Plant-derived cellulose scaffolds offer potential as biocompatible substrates for osteoblast growth and differentiation.

Purpose of the Study:

  • To generate bone-like tissue using decellularized plant scaffolds seeded with osteogenically stimulated human induced pluripotent stem cells (hiPSCs).
  • To evaluate the efficacy of different plant scaffolds in supporting osteogenesis and bone formation.
  • To assess the potential of the generated bone-like tissue for in vivo bone regeneration.

Main Methods:

  • Decellularization of various plant scaffolds.
  • Seeding of hiPSCs onto scaffolds, with osteogenic stimulation.
  • Assessment of cellular marker expression and calcium deposition.
  • Implantation of generated bone-like tissue into a rat calvarial defect model.

Main Results:

  • Apple scaffolds with 300 μm pores demonstrated optimal performance.
  • Successful generation of mineralized bone-like tissue in vitro.
  • The generated tissue facilitated calcified tissue formation in a rat calvarial defect model.
  • Scaffold pore regularity and size significantly influenced bone production.

Conclusions:

  • Decellularized plant cellulose scaffolds, particularly apple-derived ones, are effective for generating bone-like tissue from hiPSCs.
  • This approach shows promise for bone tissue engineering and regenerative medicine applications.
  • Optimizing scaffold pore characteristics is crucial for efficient bone mineralization.