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Evaluating MERS-CoV Entry Pathways.

Enya Qing1, Michael P Hantak1, Gautami G Galpalli1

  • 1Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 29, 2019
PubMed
Summary
This summary is machine-generated.

Middle East respiratory syndrome coronavirus (MERS-CoV) entry mechanisms are adaptable, utilizing diverse host factors for cell penetration. Understanding these pathways aids in developing effective antiviral strategies against MERS-CoV and other coronaviruses.

Keywords:
Coronavirus (CoV)EndosomeHR2 peptideIFITM3Middle East respiratory syndrome (MERS)ProteaseProtease inhibitorPseudovirusSpike (S)TMPRSS2TransfectionViral entryVirus concentrationVirus purification

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Area of Science:

  • Virology
  • Cell Biology
  • Infectious Diseases

Background:

  • Middle East respiratory syndrome coronavirus (MERS-CoV) is a significant zoonotic pathogen.
  • MERS-CoV exhibits a broad host range due to adaptable cell entry mechanisms.

Purpose of the Study:

  • To detail methodologies for distinguishing MERS-CoV cell entry pathways.
  • To highlight key factors, inhibitors, and viral determinants influencing MERS-CoV cell entry routes.

Main Methods:

  • Utilized established methodologies to investigate MERS-CoV cell entry.
  • Focused on analyzing virus-host cell interactions, including receptor binding and protease activation.

Main Results:

  • MERS-CoV employs adaptable cell entry, binding to host carbohydrates and protein receptors.
  • Diverse host proteases facilitate MERS-CoV membrane fusion and genome delivery.
  • Entry can occur at the cell surface or within endosomes.

Conclusions:

  • The described methods effectively differentiate MERS-CoV cell entry pathways.
  • These methodologies are applicable to other coronaviruses (CoVs) and protease-dependent viruses.