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Updated: Jan 1, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

DNA Origami Post-Processing by CRISPR-Cas12a.

Qiancheng Xiong¹, Chun Xie¹, Zhao Zhang¹

¹Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA.

Angewandte Chemie (International Ed. in English)

|December 29, 2019

Summary

This summary is machine-generated.

Researchers developed a new enzymatic method using CRISPR-Cas12a to reconfigure DNA-origami nanostructures. This technique precisely removes single-stranded DNA, enabling complex designs and transformations for advanced nanomaterials and biotechnology applications.

Keywords:

CRISPR-Cas DNA nanotechnology DNA origami molecular devices self-assembly

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Published on: December 11, 2020

Area of Science:

Nanotechnology
Biotechnology
Molecular Biology

Background:

DNA-origami enables customizable nanostructures with broad applications.
Separating components in continuous scaffold DNA-origami structures is a significant challenge, limiting modularity.
Existing methods for DNA-origami manipulation are often complex and lack precision.

Purpose of the Study:

To develop a novel enzymatic method for reconfiguring DNA-origami structures.
To overcome limitations in modularity and functionality of DNA-origami devices.
To enable the creation of intricate and dynamic nanostructures.

Main Methods:

Utilized CRISPR-Cas12a, a non-sequence-specific single-stranded DNA endonuclease.
Targeted and removed single-stranded DNA regions within DNA-origami architectures.
Applied the method to DNA structures with diverse geometrical and mechanical properties.

Main Results:

Successfully demonstrated a facile and selective enzymatic post-processing method for DNA-origami.
Achieved intricate nanostructures and complex structural transformations previously difficult to engineer.
Showcased the versatility of the method across various DNA structure types.

Conclusions:

The CRISPR-Cas12a based enzymatic method offers a powerful tool for DNA-origami reconfiguration.
This approach enhances the modularity and functional potential of DNA-origami devices.
The biocompatibility of Cas12a suggests future applications in intracellular nanodevices.