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Published on: July 3, 2014
Recombinant Human Milk Fat Globule-Epidermal Growth Factor 8 Attenuates Microthrombosis after Subarachnoid Hemorrhage
Jikai Wang1, Yuchun Zuo2, Kai Zhuang1
1Department of Neurosurgery, Third XiangYa Hospital, Central South University, Changsha, Hunan, China.
Background:
Microthrombosis after subarachnoid hemorrhage has an adverse effect on prognosis. Milk fat globule-epidermal growth factor 8 promotes phagocytosis of phagocytic cells and may reduce microthrombosis. This study investigated the effects of recombinant human milk fat globule-epidermal growth factor 8 on microthrombosis and neurological function after subarachnoid hemorrhage.
Methods:
Rats subarachnoid hemorrhage model was induced by intravascular puncture method. Western blot was performed to measure the expression of endogenous milk fat globule-epidermal growth factor 8 after subarachnoid hemorrhage. Microthrombosis was quantified by microthrombi count using immunohistochemistry and immunofluorescence. The neuroprotective effect of recombinant human milk fat globule-epidermal growth factor 8 administration was evaluated by modified Garcia score, beam balance, Rotarod test, and Morris water maze.
Results:
Endogenous milk fat globule-epidermal growth factor 8 protein level increased after subarachnoid hemorrhage. Microthrombosis was significantly increased in subarachnoid hemorrhage rats brain, while recombinant human milk fat globule-epidermal growth factor 8 dramatically reduced microthrombosis as well as improve short- and long- term neurobehavior after subarachnoid hemorrhage.
Conclusions:
Recombinant human milk fat globule-epidermal growth factor 8 reduces microthrombosis and improves neurological function after subarachnoid hemorrhage, which may be an effective strategy for treating subarachnoid hemorrhage.
Insights
Recombinant human milk fat globule-epidermal growth factor 8 (MFGE8) reduces microthrombosis and improves neurological function after subarachnoid hemorrhage (SAH). This suggests MFGE8 is a potential therapeutic strategy for SAH.
Area of Science:
- Neuroscience
- Biochemistry
- Pathology
Background:
- Microthrombosis following subarachnoid hemorrhage (SAH) negatively impacts patient prognosis.
- Milk fat globule-epidermal growth factor 8 (MFGE8) enhances phagocytosis and may mitigate microthrombosis.
Purpose of the Study:
- To investigate the therapeutic potential of recombinant human MFGE8 in mitigating microthrombosis and improving neurological outcomes post-SAH.
Main Methods:
- SAH was induced in a rat model.
- Western blot analyzed endogenous MFGE8 expression.
- Microthrombosis was quantified via immunohistochemistry and immunofluorescence.
- Neurobehavioral recovery was assessed using Garcia score, beam balance, Rotarod, and Morris water maze tests.
Main Results:
- Endogenous MFGE8 levels increased post-SAH.
- SAH rats exhibited significantly increased brain microthrombosis.
- Recombinant human MFGE8 administration markedly reduced microthrombosis.
- MFGE8 treatment improved both short- and long-term neurobehavioral function in SAH rats.
Conclusions:
- Recombinant human MFGE8 effectively reduces microthrombosis and enhances neurological function after SAH.
- MFGE8 presents a promising therapeutic strategy for managing subarachnoid hemorrhage.
