Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

4.7K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BMP4 signaling regulates the expression of signals and transcription factors for coordinated cytodifferentiation in the murine ureter.

The Biochemical journal·2026
Same author

Author Correction: Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote T<sub>H</sub>17 differentiation.

Nature immunology·2026
Same author

Interplay of SHH, WNT and BMP4 signaling regulates the development of the lamina propria in the murine ureter.

Development (Cambridge, England)·2025
Same author

CIROZ is dispensable in ancestral vertebrates but essential for left-right patterning in humans.

American journal of human genetics·2025
Same author

Splenic fibroblasts control marginal zone B cell movement and function via two distinct Notch2-dependent regulatory programs.

Immunity·2024
Same author

Use of antibodies against Epstein-Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformation.

Archives of virology·2024

Related Experiment Video

Updated: Jan 1, 2026

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

10.9K

CFAP43 modulates ciliary beating in mouse and Xenopus.

Ev Rachev1, Karin Schuster-Gossler1, Franziska Fuhl2

  • 1Institute for Molecular Biology, OE5250, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Developmental Biology
|December 30, 2019
PubMed
Summary

The CFAP43 gene is crucial for motile cilia function, impacting airway clearance, male fertility, and brain development. Loss of CFAP43 leads to mucus buildup, infertility, and hydrocephalus.

Keywords:
CFAP43CiliaCiliogenesisHydrocephalusMale infertilityMouseMucociliary clearanceXenopus laevis

More Related Videos

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo
08:00

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

Published on: July 13, 2015

12.5K
Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
08:44

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Published on: February 21, 2021

4.7K

Related Experiment Videos

Last Updated: Jan 1, 2026

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

10.9K
Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo
08:00

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

Published on: July 13, 2015

12.5K
Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
08:44

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Published on: February 21, 2021

4.7K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Malfunctioning motile cilia cause human diseases like impaired airway clearance, infertility, and hydrocephalus.
  • The transcription factor FOXJ1 is essential for motile ciliogenesis.

Purpose of the Study:

  • To characterize the evolutionary conserved Cfap43 gene and its role in motile cilia function.
  • To investigate the function of CFAP43 in various biological processes using loss-of-function models.

Main Methods:

  • Loss-of-function experiments were conducted in mouse and Xenopus laevis models.
  • Analysis included gene expression, protein localization, ciliary function assays, and phenotypic observations.

Main Results:

  • CFAP43 localizes to the ciliary axoneme and regulates tracheal cilia beating frequency.
  • Loss of CFAP43 resulted in nasal mucus accumulation, male infertility, and early-onset hydrocephalus.
  • Xenopus models showed impaired motile cilia function in larval epidermis.

Conclusions:

  • CFAP43 is essential for motile cilia function in airway epithelia, male reproductive tract, and brain development.
  • This study confirms CFAP43's role in male infertility and highlights its importance in mucus clearance and preventing hydrocephalus.