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Updated: Jan 1, 2026

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Published on: November 5, 2019
Validation of methylene blue viability staining with the emerging pathogen Candida auris
Ryan A Parker1, Kyle T Gabriel1, Kayla Graham1
1BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA.
Abstract:
Methylene blue viability staining has been traditionally used to assess viability of Saccharomyces cerevisiae in brewing and wine making. Here, this method was tested and validated with the emerging fungal pathogen Candida auris to determine if this species would also deferentially stain, which could provide utility in assessing microbial control and disinfectant efficacy.
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