Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jan 1, 2026

Optogenetic Stimulation of the Auditory Nerve

Optogenetic Stimulation of the Auditory Nerve

Published on: October 8, 2014

Sonogenetic Modulation of Cellular Activities Using an Engineered Auditory-Sensing Protein.

Yao-Shen Huang¹, Ching-Hsiang Fan², Ning Hsu¹

¹Institute of Molecular Medicine , National Tsing Hua University , Hsinchu 300 , Taiwan.

|December 31, 2019

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modulation of Cilia Motility by Vortex-Ultrasound-Induced Shear Stress.

ACS nano·2026

Same author

Contribution of HMGB1 to keratinocyte inflammation in recessive dystrophic epidermolysis bullosa.

JID innovations : skin science from molecules to population health·2026

Same author

Detection of mutant cancer-derived circRNAs in extracellular vesicles by rolling circle amplification.

Molecular therapy. Nucleic acids·2026

Same author

High level dynein impairs mitochondrial distribution and differentiation of rhabdomyosarcoma cells.

iScience·2026

Same author

Cell Cycle-Specific Regulation of Centrosome Clustering Dynamics in Cancer Cells by the Multifunctional Kinesin HSET.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Enhancing prestin oligomerization for superior sonogenetics.

Theranostics·2026

Researchers developed a sonogenetic system using an engineered protein to control genetically modified cells with ultrasound. This breakthrough enables noninvasive stimulation of deep brain neurons for potential therapeutic applications.

Area of Science:

Biotechnology
Cellular Biology
Neuroscience

Background:

Genetically modified cells can be controlled remotely by external stimuli.
Developing noninvasive methods for cellular manipulation is crucial for research and therapy.

Purpose of the Study:

To establish a sonogenetic approach for manipulating cell activity using focused ultrasound.
To engineer an ultrasound-responsive protein for sensing and responding to ultrasound stimulation.

Main Methods:

Engineered an auditory-sensing protein (prestin) with specific amino acid substitutions (N7T, N308S).
Expressed the engineered prestin in mammalian cells and tested responses to ultrasound.
Applied pulsed ultrasound to stimulate target neurons in deep brain regions of mice.

Keywords:

Sonogenetics focused ultrasound mammalian cells neuromodulation

More Related Videos

Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Published on: January 20, 2015

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Published on: April 17, 2016

Related Experiment Videos

Last Updated: Jan 1, 2026

Optogenetic Stimulation of the Auditory Nerve

Optogenetic Stimulation of the Auditory Nerve

Published on: October 8, 2014

Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Published on: January 20, 2015

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Published on: April 17, 2016

Main Results:

Transfected mammalian cells expressing prestin(N7T, N308S) gained the ability to sense ultrasound.
Low-frequency, low-pressure ultrasound efficiently evoked cellular calcium responses.
Pulsed ultrasound noninvasively stimulated deep brain neurons expressing the engineered protein.

Conclusions:

An engineered auditory-sensing protein can confer ultrasound sensitivity to mammalian cells.
Sonogenetic tools offer novel strategies for noninvasive deep tissue therapies.