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Updated: Jan 1, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

A solid-phase transfection platform for arrayed CRISPR screens.

Özdemirhan Serçin¹, Sabine Reither², Paris Roidos¹

¹BioMed X Innovation Center, Heidelberg, Germany.

Molecular Systems Biology

|December 31, 2019

Summary

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This summary is machine-generated.

Arrayed CRISPR screens offer a new way to study cell behaviors. This study introduces a solid-phase transfection platform for flexible, high-throughput CRISPR screens, enabling new therapeutic target discovery.

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Pooled CRISPR screens are limited for certain cellular phenotypes.
Arrayed CRISPR screens offer an alternative but require efficient delivery methods.

Purpose of the Study:

To develop a solid-phase transfection platform for arrayed CRISPR-based genetic screens.
To demonstrate the platform's utility in studying oncogene addiction and editing primary cells.

Main Methods:

Development of a solid-phase transfection platform for arrayed CRISPR screens.
Delivery of guide RNAs and Cas9/guide RNA ribonucleoprotein complexes.
Application of the platform for high-throughput screening and primary cell editing.

Main Results:

Keywords:

CRISPR/Cas9 arrayed screens gRNA/RNP delivery solid phase transfection platform

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

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Efficient gene knockout demonstrated in various cell lines using the platform.
Successful adaptation of the platform for high-throughput screening.
Demonstrated applicability to oncogene addiction studies and human primary cell editing.

Conclusions:

The developed platform enables flexible and efficient arrayed CRISPR screening.
This method facilitates high-throughput analysis of cellular phenotypes and oncogene addiction.
The platform supports rapid testing of targeted therapies using primary human cells.