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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Updated: Jan 1, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Automated Counterflow Centrifugal System for Small-Scale Cell Processing.

Anqi Li¹, Stephen Wilson², Ian Fitzpatrick²

¹The Ritchie Centre, Hudson Institute of Medical Research; Department of Obstetrics and Gynaecology, Monash University.

Journal of Visualized Experiments : Jove

|December 31, 2019

Summary

This summary is machine-generated.

A new automated cell processing device offers a scalable solution for buffer exchange in gene and cell therapy manufacturing. This system improves efficiency and cell recovery compared to manual methods.

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Area of Science:

Biotechnology
Cellular Manufacturing
Process Engineering

Background:

Commercializing gene and cell therapies necessitates cost-effective and scalable manufacturing.
Buffer exchange and product concentration are critical but often manually intensive steps early in development.
Manual methods like dead-end centrifugation are laborious, expensive, and hinder scalability.

Purpose of the Study:

To introduce a novel automated cell processing device for small- to medium-scale applications.
To bridge the gap between manual cell processing and large-scale automated manufacturing.
To provide a versatile protocol adaptable to various cell types and processes.

Main Methods:

Development of a new automated cell processing device.
Modification of flow rate and centrifugation speed for different cell types and processes.
Comparison of automated processing with manual dead-end centrifugation.

Main Results:

High cell recovery rates were achieved using the automated protocol.
Processing times were significantly shorter compared to manual methods.
Recovered cells maintained their proliferation capabilities post-processing.

Conclusions:

The developed device offers an efficient and scalable solution for cell processing steps like buffer exchange.
It serves as a modular component within closed manufacturing systems for cell formulation and cryopreservation.
This automation addresses the limitations of manual processing in early-stage cell therapy development.