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Early urine proteome changes in an implanted bone cancer rat model
Ting Wang1, Lujun Li1, Weiwei Qin1,2
1Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing 100875, China.
Bone Reports
|December 31, 2019
Summary
Urine analysis detected significant protein changes in rats with early bone cancer, even before imaging revealed lesions. These urinary protein profiles offer potential for early tumor bone metastasis detection.
Area of Science:
- Oncology
- Biochemistry
- Proteomics
Background:
- Bone metastasis is a significant complication of cancer.
- Early detection of bone metastasis is crucial for effective treatment.
- Current diagnostic methods like Computed Tomography (CT) may not detect early-stage bone lesions.
Purpose of the Study:
- To investigate the potential of urinary proteomic analysis for early detection of bone metastasis.
- To identify specific protein biomarkers in urine associated with early-stage bone cancer.
Main Methods:
- Walker 256 cancer cells were implanted into rat tibiae.
- Urine samples were collected on days 3, 5, 7, and 13 post-implantation.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with label-free quantification was used for protein analysis.
Main Results:
- Twenty-five urinary proteins showed significant changes in rats with implanted bone cancer compared to controls, even without visible CT lesions.
- Seven of these differentially expressed proteins are linked to tumor bone metastasis.
- Gene Ontology (GO) analysis indicated involvement in immune responses (day 3), mineral absorption (day 7), and vitamin D/calcium binding (day 13).
Conclusions:
- Urinary proteomic profiling can sensitively detect early-stage bone cancer changes.
- These findings suggest potential urine biomarkers for early diagnosis of tumor bone metastasis.
- Further research into these urinary proteins could lead to novel diagnostic tools.
