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Updated: Dec 31, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Quantification of aromatic amines derived from azo colorants in textile by ion-pairing liquid chromatography tandem
Ádám Tölgyesi1, Virender K Sharma2
1ÉMI-TÜV SÜD Ltd., Dugonics utca 11, 1043 Budapest, Hungary.
Abstract:
Azo dyes can metabolize back to precursor aromatic amines (arylamines), which are potentially carcinogenic. The ISO 14362-1:2017 standard requires the determination of the arylamines, produced from textile samples, preferably by using the gas chromatography mass spectrometric (GC-MS) method. This paper presents an ion-pairing high performance liquid chromatography tandem mass spectrometric (LC-MS/MS) method for determining aromatic amines, derived from azo colorants, in both natural and synthetic textiles. The separation enables adequate apparent retention factor (k' > 2.1 for the most hydrophilic compounds) and has appropriate sensitivity without sample clean-up procedure. The background matrix constituents influence the quantification in even a 100-fold diluted sample, therefore, calibration requires background compensation. The method allows fast confirmation and quantification of twenty-five arylamines in complex matrices of textiles. The method was validated with success and used to both natural and synthetic textile proficiency test (PT) samples.
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