Chitosan-toluidine blue beads for purification of fucoidans

A simple and green method was developed to purify fucoidans from their crude extracts. The new method utilizes a genipin-crosslinked chitosan beads as a support matrix for toluidine blue (TB). The modification of the mostly composed of d-glucosamine polymer was performed in one-step reaction to improve its mechanical stability and affinity to fucoidans. The adsorption kinetics and isotherm were investigated, which showed a maximum loading capacity (q_max) of 137.8 mg fucoidans/g wet beads. Moreover, the modified chitosan-TB beads were applied for purification of fucoidans from Fucus vesiculosus crude extract at different pH values, pH 1.0 and pH 6.0, producing two fractions: FC_1 and FC_6, respectively. The fractions were then characterized in comparison with crude and Sigma-Aldrich® purified product by FTIR and elemental analysis. The new method produced beads with higher loading capacity and used a natural crosslinker compared to the previously-reported methods.