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Published on: August 10, 2018
Plasma exosomal miR-106a-5p expression in myasthenia gravis
Hongyan Xu1, Zhuohua Bao1, Daye Liang1
1Department of Neurology, the First Affiliated Hospital of Guangxi Medical University, Guangxi, China.
Background:
Exosomal miRNA expression for myasthenia gravis (MG) has not been studied.
Methods:
Plasma samples from 92 patients with MG and 49 age-matched healthy controls (HCs) were screened (by means of deep sequencing and quantitative real-time polymerase chain reaction) for differentially expressed exosomal microRNA (miRNAs). miR-106a-5p was chosen to further verify because it is reportedly involved in MG pathogenesis. Spearman's correlation analysis was used to assess correlations between candidate miRNAs and patient quantitative MG scores (QMGSs). Area under the curve (AUC) of the receiver operating characteristic analysis was used to evaluate the diagnostic accuracy of the identified miRNAs for MG.
Results:
miR-106a-5p levels were significantly decreased in MG patients compared with HCs, and were associated with patient QMGS. The AUC values for hsa-miR-106a-5p were 0.728 and 0.813 in ocular and generalized MG patients, respectively.
Conclusions:
Exosomal miR-106a-5p was expressed differently in different types of MG and was associated with MG severity.
Insights
Exosomal microRNA (miRNA) levels, specifically miR-106a-5p, are altered in myasthenia gravis (MG) patients. This finding suggests exosomal miR-106a-5p may serve as a biomarker for MG diagnosis and severity.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Exosomal microRNA (miRNA) expression has not been previously investigated in myasthenia gravis (MG).
- Understanding exosomal miRNA profiles in MG is crucial for identifying novel biomarkers.
Purpose of the Study:
- To investigate the expression of exosomal miRNAs in patients with myasthenia gravis (MG).
- To evaluate the diagnostic potential of identified exosomal miRNAs for MG.
- To assess the correlation between exosomal miRNA levels and MG severity.
Main Methods:
- Deep sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to screen plasma samples from 92 MG patients and 49 healthy controls (HCs).
- miR-106a-5p was selected for further verification due to its reported involvement in MG pathogenesis.
- Spearman's correlation and receiver operating characteristic (ROC) analysis were employed to assess correlations and diagnostic accuracy.
Main Results:
- Exosomal miR-106a-5p levels were significantly decreased in MG patients compared to HCs.
- miR-106a-5p levels correlated with the quantitative MG score (QMGS), indicating an association with disease severity.
- The area under the curve (AUC) for hsa-miR-106a-5p was 0.728 for ocular MG and 0.813 for generalized MG, demonstrating diagnostic potential.
Conclusions:
- Exosomal miR-106a-5p exhibits differential expression in various types of MG.
- The study identifies exosomal miR-106a-5p as a potential biomarker associated with MG type and disease severity.
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