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Updated: Dec 31, 2025
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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Site-directed RNA editing (SDRE): Off-target effects and their countermeasures
Shaoshuai Mao1, Yajing Liu1, Shisheng Huang1
1School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China; CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Journal of Genetics and Genomics = Yi Chuan Xue Bao
|January 1, 2020
Summary
Site-directed RNA editing (SDRE) is a powerful genome editing tool. Strategies are being developed to overcome off-target effects, a key challenge for its clinical use.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- Site-directed RNA editing (SDRE) is a rapidly advancing field with significant potential in basic research and clinical applications.
- The development of SDRE tools has accelerated, highlighting its growing importance in genome editing technologies.
Purpose of the Study:
- To discuss diverse strategies for combating off-target effects in site-directed RNA editing.
- To review lessons from published studies and ongoing research to improve SDRE precision.
Main Methods:
- Literature review of published studies on SDRE.
- Analysis of ongoing research focused on mitigating off-target editing.
Main Results:
- Off-target effects remain a significant challenge hindering the clinical application of SDRE.
- Various strategies have been explored to enhance the specificity of SDRE tools.
Conclusions:
- Site-directed RNA editing is an emerging technology with substantial challenges and opportunities.
- Continued research into minimizing off-target effects is crucial for realizing the full potential of SDRE in medicine and research.
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