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RAD51 is a potential marker for prognosis and regulates cell proliferation in pancreatic cancer

Xiaomeng Zhang1,2, Ningyi Ma3,4, Weiqiang Yao1,2

  • 11Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032 China.

Cancer Cell International
|January 1, 2020
PubMed
Abstract

Insights

RAD51 promotes pancreatic cancer cell proliferation and aerobic glycolysis by regulating HIF1α. Higher RAD51 levels correlate with worse patient survival, indicating its potential as a predictive and therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • DNA damage and repair pathways are key targets in cancer therapy.
  • RAD51 (RECA) is crucial for homologous recombination and implicated in tumor progression and chemoresistance.
  • The role of RAD51 in pancreatic cancer remains under-investigated.

Purpose of the Study:

  • To investigate the role of RAD51 in pancreatic cancer progression.
  • To explore the relationship between RAD51, KRAS, and aerobic glycolysis in pancreatic cancer.
  • To assess RAD51 as a prognostic marker for pancreatic cancer survival.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) dataset for RAD51 expression and patient survival.
  • Lentivirus-mediated knockdown of RAD51 and KRAS in pancreatic cancer cells.
  • Quantitative real-time PCR and western blot to confirm knockdown efficacy.
  • Assays to evaluate cell proliferation and aerobic glycolysis, including HIF1α levels.

Main Results:

  • Elevated RAD51 expression is linked to poorer overall survival in pancreatic cancer patients.
  • RAD51 knockdown reduced pancreatic cancer cell proliferation and ROS production.
  • KRAS/MEK/ERK pathway activation positively regulates RAD51 expression.
  • RAD51 was identified as a key regulator of aerobic glycolysis via HIF1α.

Conclusions:

  • RAD51 plays a significant role in pancreatic cancer, influencing proliferation and aerobic glycolysis.
  • RAD51 may serve as a predictive biomarker for patient outcomes in pancreatic cancer.
  • Targeting RAD51 presents a potential therapeutic strategy for pancreatic cancer.
Keywords:
Aerobic glycolysisKRASPancreatic cancerPrognosisRAD51

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