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Updated: Dec 31, 2025
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Development, characterization, and cross-amplification of 17 microsatellite markers for Filipendula vulgaris
Dijana Čortan1, Karol Krak1,2, Petr Vít1,2
1Faculty of Environmental Sciences Czech University of Life Sciences Prague Kamýcká 129 Praha 6-Suchdol CZ-165 00 Czech Republic.
Premise:
Polymorphic microsatellite markers were developed as a tool for genetic investigations of Filipendula vulgaris (Rosaceae) and related species.
Methods And Results:
Seventeen new polymorphic microsatellite markers were developed for F. vulgaris using the Illumina MiSeq platform. Polymorphism of the 17 loci was tested in three populations. We identified a total of 203 alleles, ranging from four to 19 per locus, with levels of observed and expected heterozygosity ranging from 0.267 to 1.000 and 0.461 to 0.899, respectively. Primers were also tested for cross-amplification in three related species. Seven loci successfully cross-amplified in F. camtschatica and F. ulmaria, whereas we detected positive cross-amplification in only three loci in Geum urbanum.
Conclusions:
The newly developed microsatellite primers will serve as useful genetic tools for further population genetic studies on F. vulgaris and related species.
