Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A pharmacologic study of the relationship between lymphocyte function and surface antigen expression.

M L Bliven1, A C Cunningham, I G Otterness

  • 1Pfizer Central Research, Groton, CT 06340.

Agents and Actions
|August 1, 1988
PubMed
Summary

Lymphocyte function changes occur before surface marker alterations. Immunosuppressant drugs can normalize these markers, indicating they are late indicators of immune response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measuring interoception: The phase adjustment task.

Biological psychology·2021
Same author

Functional restoration for chronic low back pain: Changes in depression, cognitive distortion, and disability.

Journal of occupational rehabilitation·2013
Same author

Allometric relationships between knee cartilage volume, thickness, surface area and body dimensions.

Osteoarthritis and cartilage·2007
Same author

Role of the mucosal integrin alpha(E)(CD103)beta(7) in tissue-restricted cytotoxicity.

Clinical and experimental immunology·2007
Same author

Women have thinner cartilage and smaller joint surfaces than men after adjustment for body height and weight.

Osteoarthritis and cartilage·2007
Same author

A GPI-anchored co-receptor for tissue factor pathway inhibitor controls its intracellular trafficking and cell surface expression.

Journal of thrombosis and haemostasis : JTH·2006

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • The interplay between lymphocyte function and surface marker expression remains incompletely understood.
  • Investigating these relationships is crucial for comprehending immune responses and the effects of immunosuppressive therapies.

Purpose of the Study:

  • To elucidate the relationship between lymphocyte surface markers and cellular function.
  • To examine how immunosuppressant therapy influences these markers and functional activity.

Main Methods:

  • Utilized flow cytometry (FACS) to analyze spleen cells from immunized BALB/c mice.
  • Assessed changes in lymphocyte surface markers (Thy 1, Ig, Lyt 2) following immunization and immunosuppressant treatment (azathioprine, cyclophosphamide).
  • Correlated marker expression with functional activity of cytotoxic T cells.

Related Experiment Videos

Main Results:

  • Immunization led to decreased Thy 1+ and increased Ig+ spleen cells, while Lyt 2+ cell populations remained unchanged.
  • Low-dose immunosuppressants did not alter cell surface markers, but higher doses normalized immunization-induced marker changes.
  • Functional activity of cytotoxic T cells increased post-immunization without corresponding changes in Lyt 2+ cell percentage or staining intensity.

Conclusions:

  • Lymphocyte functional changes precede alterations in cell surface antigen expression.
  • Functional immune shifts can occur independently of detectable changes in surface markers.
  • Cell surface markers serve as late indicators of underlying functional immune dynamics.