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Related Experiment Videos

Deconstructing and Reconstructing Cheese Rind Microbiomes for Experiments in Microbial Ecology and Evolution.

Casey M Cosetta1, Benjamin E Wolfe1

  • 1Department of Biology, Tufts University, Medford, Massachusetts.

Current Protocols in Microbiology
|January 1, 2020
PubMed
Summary

Researchers developed methods to study cheese rind microbial communities, enabling controlled experiments to understand microbiome diversity and interactions. This approach can be applied to other fermented foods, creating new model microbiomes for research.

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Area of Science:

  • Microbiology
  • Microbial Ecology
  • Fermentation Science

Background:

  • Cheese rinds harbor complex microbial communities that serve as valuable models for studying microbiome dynamics.
  • Understanding the mechanisms governing microbial diversity is crucial for various applications, including food science and biotechnology.

Purpose of the Study:

  • To present optimized methods for deconstructing and reconstructing cheese rind microbial communities in vitro.
  • To enable controlled laboratory manipulations for investigating microbial interactions and community assembly.

Main Methods:

  • Isolation and culturing of diverse bacterial, yeast, and fungal species from cheese rinds using standard and specialized media (e.g., cheese curd agar).
  • Identification of isolates via 16S and ITS sequencing.
Keywords:
bacteriacheesefungimicrobial ecologymicrobiome

Related Experiment Videos

  • Reconstruction of synthetic microbial communities and assessment of interactions through transcriptomics and transposon mutagenesis screens.
  • Functional assays including pH measurement and pigment production analysis.

    • Main Results:

    • Demonstrated successful isolation and culturing of a wide range of cheese rind microbes.
    • Established protocols for reconstructing diverse microbial communities in a laboratory setting.
    • Developed techniques to pinpoint mechanisms of microbial interactions within these communities.

    Conclusions:

    • The described methodology provides a tractable system for studying microbial community assembly and function.
    • This approach is adaptable to other fermented food systems, facilitating the development of novel model microbiomes.
    • The study offers a robust framework for dissecting the complex interplay of microbes in fermented environments.