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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Mini-Disk Capillary Array Coupling with LAMP for Visual Detection of Multiple Nucleic Acids using Genetically

Rong Li1, Jianwei Chen2, Xiujie Zhang3

  • 1Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology , Shanghai Jiao Tong University , Shanghai 200240 , China.

Journal of Agricultural and Food Chemistry
|January 1, 2020
PubMed
Summary

We developed a novel multiplex nucleic acid testing (NAT) system, mDC-LAMP, for convenient, low-cost, and portable on-site monitoring. This system offers high specificity, sensitivity, and throughput for various applications.

Keywords:
genetically modified organismloop-mediated isothermal amplificationmini-disk capillary arraymultiplex nucleic acid testingvisual detection

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Analytical Chemistry

Background:

  • Multiplex nucleic acid testing (NAT) systems are crucial for food safety, environmental monitoring, and molecular diagnostics.
  • Current systems often lack convenience, portability, and affordability, hindering on-site applications.

Purpose of the Study:

  • To develop a novel, low-cost, portable system for visual monitoring of multiple nucleic acids.
  • To evaluate the performance and applicability of the developed system for multiplex nucleic acid detection.

Main Methods:

  • Development of a mini-disk capillary array (mDC) system integrated with visual loop-mediated isothermal amplification (LAMP).
  • Incorporation of a quick DNA extraction method for rapid sample preparation.
  • Evaluation using genetically modified (GM) content analysis as a model application.

Main Results:

  • The mDC-LAMP system demonstrated high specificity with no cross-contamination.
  • Achieved high sensitivity with a limit of detection of 25 copies/reaction.
  • Showcased high throughput capabilities with flexible channel sensors and ease of fabrication.

Conclusions:

  • mDC-LAMP is a competitive and cost-effective solution for on-site multiplex nucleic acid testing.
  • The system's ease of fabrication, operation, and suitable performance make it ideal for field applications.
  • This technology advances portable diagnostics for food safety, environmental monitoring, and clinical applications.