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Bovine Lactoferrin Quantification in Dairy Products by a Simple Immunoaffinity Magnetic Purification Method Coupled
Jingjing Pang1, Qianqian Xiao1, Han Yan1
1School of Pharmacy , Weifang Medical University , 7166 Baotong West Street , Weifang , Shandong 261053 , China.
Abstract:
This study described a simple, specific, and sensitive method using immunoaffinity magnetic purification coupled with high-performance liquid chromatography-fluorescence (HPLC-FL) detection for determination of bovine lactoferrin (bLf) in dairy products. BLf was selectively extracted from dairy products using immunoaffinity beads and then detected by HPLC-FL with its intrinsic fluorescence. During the analysis, standard solutions of bLf were pretreated with Tween 20, an anti-adsorptive agent, for blocking the nonspecific binding of bLf to polypropylene tubes. The calibration curve was linear over the range of 0.8-30 μg mL-1. The validated method was successfully applied to measure bLf at the intact level in dairy products.
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